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      產品資料
      產品[

      CRL1746ST豬睪丸細胞系

      ]資料
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      產品名稱: CRL1746ST豬睪丸細胞系
      產品型號: CRL-1746 ST 豬睪丸細胞系
      產品廠商: 美國標準生物品收藏中心(ATCC)
      產品文檔: 無相關文檔


      簡單介紹

      CRL-1746 ST 豬睪丸細胞系,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|。細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*優培養條件!


      CRL1746ST豬睪丸細胞系 的詳細介紹

      CRL-1746 ST 豬睪丸細胞系

      ST (ATCC® CRL-1746?)
      Organism  Sus scrofa, pig 
      Tissue  testis
      Product Format  frozen 
      Morphology  fibroblast 
      Culture Properties  adherent 
      Biosafety Level  1 
      Disease  Normal 
      Age  80 to 90 days gestation 
      Gender  male 
      Applications  This line is useful for the propagation, assay and isolation of PPV, TGE and swine enteroviruses.
      Storage Conditions  liquid nitrogen vapor phase 
      Clinical Data  male
      Comments  This line is useful for the propagation, assay and isolation of PPV, TGE and swine enteroviruses.
      Complete Growth Medium  The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
       
      Subculturing  Protocol:
      1.Remove and discard culture medium.
      2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
      3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
      4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
      5.Add appropriate aliquots of the cell suspension to new culture vessels.
      6.Incubate cultures at 37°C.
      Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
      Medium Renewal: 2 to 3 times per week
      Cryopreservation  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
      Storage temperature: liquid nitrogen vapor phase
      Culture Conditions  Atmosphere: air, 95%; carbon dioxide (CO2), 5%
      Temperature: 37°C
      Name of Depositor  SJ Wessman 
      References  McClurkin AW, Norman JO. Studies on transmissible gastroenteritis of swine. II. Selected characteristics of a cytopathogenic virus common to five isolates from transmissible gastroenteritis. Can. J. Comp. Med. Vet. Sci. 30: 190-198, 1966. PubMed: 4224292 
       

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