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      產品資料
      產品[

      CCL-33 PK-15 豬腎細胞系

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      產品名稱: CCL-33 PK-15 豬腎細胞系
      產品型號: CCL-33
      產品廠商: 美國標準生物品收藏中心(ATCC)
      產品文檔: 無相關文檔


      簡單介紹

      CCL-33 PK-15 豬腎細胞系;ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*優培養條件!


      CCL-33 PK-15 豬腎細胞系 的詳細介紹
      CCL-33 PK-15 豬腎細胞系
      ATCC® Number: CCL-33™    Price: $355.00
      Designations: PK(15)
      Depositors:  Cutter Laboratories, Inc.
      Biosafety Level: 1
      Shipped: frozen
      Medium & Serum: See Propagation
      Growth Properties: adherent
      Organism: Sus scrofa (pig)
      Morphology: epithelial

      Source: Organ: kidney
      Disease: normal
      Cellular Products: plasminogen activator; keratin
      Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
       
      Applications: transfection host (Roche FuGENE® Transfection Reagents)
      Virus Resistance: poliovirus 2
      Age: *****
      Comments: The presence of a porcine papovavirus in PK(15) cells has been reported in cells obtained from multiple sources including the ATCC . [53398] [53399]
      The Foreign Animal Disease Diagnostic Laboratory of the US Department of Agriculture has determined that ATCC CCL-33 is not infected with Hog cholera virus or African swine fever virus, and uses this line to screen for those viruses. [105588]
      The cell line harbors an endogenous C-type retrovirus. [26185] [53399] [56104]
      The cells are positive for porcine circovirus (PCV) antigens.
      The cells are positive for keratin by immunoperoxidase staining.
      Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      Temperature: 37.0°C
      Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
      Medium Renewal: 2 to 3 times per week
      Rinse the cell sheet 2 times with fresh 0.25% trypsin, 0.03% EDTA solution, remove trypsin and allow the culture to stand at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
      Preservation: culture medium 95%; DMSO, 5%
      Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
      recommended serum:ATCC 30-2020
      References: 22988: Dulac GC, Afshar A. Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs. Can. J. Vet. Res. 53: 431-433, 1989. PubMed: 2686830
      26184: Pirtle EC, Woods LK. Cytogenetic alterations in swine kidney cells persistently infected with hog cholera virus and propagated with and without antiserum in the medium. Am. J. Vet. Res. 29: 153-164, 1968. PubMed: 4965860
      26185: Armstrong JA, et al. C-type virus particles in pig kidney cell lines. J. Gen. Virol. 10: 195-198, 1971. PubMed: 4324256
      53398: Newman JT, Smith KO. Characteristics of a swine papovavirus. Infect. Immun. 5: 961-967, 1972. PubMed: 4344097
      53399: Tumilowicz JJ, et al. Concurrent replication of a papovavirus and a C-type virus in the CCL 33 porcine cell line. In Vitro 15: 922-928, 1979. PubMed: 232060
      56104: Todaro GJ, et al. Characterization of a type C virus released from the porcine cell line PK(15). Virology 58: 65-74, 1974. PubMed: 4132403
      105588: James A House, personal communication
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