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      產品資料
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      CRL-10762 CTLA4 Ig-24 中國倉鼠卵巢細胞

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      產品名稱: CRL-10762 CTLA4 Ig-24 中國倉鼠卵巢細胞
      產品型號: CRL-10762
      產品廠商: 美國標準生物品收藏中心(ATCC)
      產品文檔: 無相關文檔


      簡單介紹

      CRL-10762 CTLA4 Ig-24 中國倉鼠卵巢細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*優培養條件!


      CRL-10762 CTLA4 Ig-24 中國倉鼠卵巢細胞 的詳細介紹
      CRL-10762 CTLA4 Ig-24 中國倉鼠卵巢細胞
      ATCC® Number: CRL-10762™    Price:  
      Designations: CTLA4 Ig-24
      Depositors:  Bristol-Myers Squibb Co.
      Biosafety Level: 1
      Shipped: frozen
      Medium & Serum: See Propagation
      Growth Properties: adherent
      Organism: Cricetulus griseus (hamster, Chinese)
      Morphology: CRL-10762 CTLA4 Ig-24 中國倉鼠卵巢細胞epithelial

      Source: Organ: ovary
      Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
       
      Gender: female
      Comments: The line was constructed by transfecting CHO cells with a construct (CTLA4Ig) containing the sequence for the extracellular domain of the human CTLA-4 gene fused to the sequence for the hinge, CH2 and CH3 regions of human IgC.gamma.1 domain.The cells express the fusion protein (CTLA4Ig).
      The cells express the fusion protein (CTLA4Ig).
      Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 0.2 mM proline, 0.001 mM methotrexate, 90%; fetal bovine serum, 10%
      Temperature: 37.0°C
      Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
      Medium Renewal: 2 to 3 times per week
      Remove medium, add fresh 0.25% trypsin solution for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
      References: 4548: Linsley PS. Chimeric CTLA4 receptor and methods for its use. US Patent 5,434,131 dated Jul 18 1995
      CRL-10762 CTLA4 Ig-24 中國倉鼠卵巢細胞
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